PROJECTS 2020/1

CERIUM OXIDE NANOPARTICLES FOR STROKE TREATMENT
LUNG FLUID STRUCTURE AND RHEOLOGY
MICROFLUIDICS APPROACH OF MUCOCILARY CLEARENCE
BIOMECHANICS OF LIVING CELLS

The projects listed in this section can be the subject of M1 or M2 master internships, doctoral thesis or postdoctorate (according to funding).
If you are interested, please contact Jean-François Berret here.

Biomechanics of living cells

Mechanotransduction describes the molecular mechanisms by which cells respond to changes in their physical environment by translating mechanical stimuli into biochemical signals. These mechanical changes can be either forces exerted on the cell from the environment or intracellular forces arising from cell responses to stiffness/topography modifications. The mechanical properties of cells are mainly determined by the cytoskeleton and nucleus, and are essential in major cell functions. Studies suggest that cancer cells acquire specific mechanical properties allowing them to deform more strongly than healthy cells.

We have reported on the viscosity of eukaryotic living cells using an active microrheological technique, where magnetic wires, embedded into cells, are being actuated remotely. It was shown that this technology is able to measure the viscosity of fluids with high accuracy, for samples down to 1 µL volume. Interestingly, the cytoplasm viscosity measured displays anomalous transient responses characterized by intermittent phases of low and high viscosity. An analysis based on mathematical finance statistical tools was performed, revealing specific intracellular relaxation times not previously disclosed. The current approach could be exploited to reveal hidden features from biological complex systems, or determine new biomarkers of cellular metabolism.

A detailed version of the project can be found here.

REFERENCES

Omar El Hamoui, Indresh Yadav, Milad Radiom, Frank Wien, Jean-Francois Berret, Johan R. C. van der Maarel, Véronique Arluison
Interactions between DNA and the Hfq Amyloid-like Region Trigger a Viscoelastic Response
Biomacromolecules 21(9), 3668–3677 (2020)
https://doi.org/10.1021/acs.biomac.0c00747
https://arxiv.org/abs/2007.14663

C. Bostoen*and J.-F. Berret*
A mathematical finance approach to the stochastic and intermittent viscosity fluctuations in living cells
Soft Matter 16, 5959 – 5969 (2020)
https://doi.org/10.1039/C9SM02534K
https://arxiv.org/abs/2005.08002

L.P.A. Thai, F. Mousseau, E.K. Oikonomou, M. Radiom and J.-F. Berret*
Effect of Nanoparticles on the Bulk Shear Viscosity of a Biomimetic Lung Surfactant
ACS Nano 14, 466 – 475 (2020)
https://doi.org/10.1021/acsnano.9b06293
https://arxiv.org/abs/1912.05853

J.-F. Berret*
Local viscoelasticity of living cells measured by rotational magnetic spectroscopy
Nature Communications 7, 10134 (2016)
https://doi.org/10.1038/ncomms10134
http://arxiv.org/abs/1512.01183

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