Microfluidics approach of mucociliary clearence
Fine airborne particulate matter is released from production facilities, construction sites and wildfire, and their inhalation is shown to be a major source of premature mortality in the world. Among them, ultra-fine particulate matter and nanoengineered materials (size < 100 nm), namely nanoparticles, cause the most adverse effects due to their accessibility to the distal ends of the lungs. Luckily, the body has defense mechanisms along the upper airways which traps and disposes of inhaled nanoparticles. This barrier is called mucociliary clearance and consists of a mucus layer covering the airway epithelium, and a continuous clearance of mucus from the airways via ciliary waves.
The objectives of the project are to explore the effect of the physical and chemical properties of nanoparticles on their adsorption kinetics, as well as the effect of trapped nanoparticles on the mucus viscoelasticity. For this, we will develop a mimic of mucociliary clearance using magnetic micropillars and a microfluidic chip. This chip will also integrate electromagnets for pillar actuation, accommodate access to mucus deposition and exposure to aerosolized nanoparticles.
A detailed version of the project can be found here. The projects listed in this section can be the subject of M1 or M2 master internships, doctoral thesis or postdoctorate (according to funding). If you are interested, please contact me.
M. Radiom*, Y. He, J. Peng, A. Baeza-Squiban, J.-F. Berret, Y. Chen*
Alveolar mimics with periodic strain and its effect on the cell layer formation
Biotechnology and Bioengineering 117(9), 2827-2841 (2020)
J. Le Digabel, N. Biais, J. Fresnais, J.-F. Berret, P Hersen and B. Ladoux*
Magnetic micropillars as a tool to govern substrate deformations
Lab Chip 11, 2630 (2011)